| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Oyster hemocytes express a proline-rich peptide displaying synergistic antimicrobial activity with a defensin
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| Autores: |
Gueguen, Yannick
Romestand, Bernard
Fievet, Julie
Schmitt, Paulina
Destoumieux Garzon, Delphine
Franck, Vandenbulcke
Philippe, Bulet
Bachere, Evelyne
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| Data: |
2009-02
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| Ano: |
2009
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| Palavras-chave: |
Antimicrobial peptide
Mollusk
Synergy
Invertebrate
Innate immunity
Pacific oyster
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| Resumo: |
A cDNA sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (AMPs) was identified in the oyster Crassostrea gigas. After release of a hydrophobic signal peptide. the resulting 37-amino acid peptide, Cg-Prp, is composed of an acidic region and a cationic proline-rich region. To evaluate the biological properties of Cg-Prp, multiple proline-rich peptides corresponding to putative processing of the full-length Cg-Prp were synthesized. A limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with Cg-Def, a defensin from C gigas. To our knowledge, this is the first evidence of synergy between a defensin and another AMP in an invertebrate. By in situ hybridization, the expression of Cg-prp was found to be restricted to hemocytes and induced following bacterial challenge. Cg-prp transcripts were also detected in hemocytes infiltrating mantle, where Cg-Def is expressed. Additionally, by immunocytochemistry, we showed that Cg-Prp or one of its variants is present in some hemocytes together with defensins. In conclusion, we described here the first proline-rich AMP from mollusk. From our study, it is likely to provide a first line of defense against bacterial invasion by acting through synergy with defensins. (C) 2008 Elsevier Ltd. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2009/publication-6232.pdf
DOI:10.1016/j.molimm.2008.07.021
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/6232/
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| Formato: |
application/pdf
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| Fonte: |
Molecular Immunology (0161-5890) (Elsevier), 2009-02 , Vol. 46 , N. 4 , P. 516-522
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| Direitos: |
2009 Elsevier
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